Khảo sát đậm độ và khả năng sinh độc tố của vi khuẩn S. aureus

TÓM TẮT KHÓA LUẬN

           
 
Staphylococcus aureus 

ThS. 
Staphylococcus aureus 
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 

 , 
S. aureus
S. aureus              
S. aureus 
   S. aureus         (Tecra
Staphylococcal Growth Medium)  (Brain Heart Infusion)
 


S. aureus  
(
S. aureus 
 
                
 


S. aureus.
S. aureus 


ABSTRACT

Staphylococcus aureus is a Gram-possitive coccus having ability to produce
enterotoxin. It is reponsible for one of the most common types of food poisoning. In
testing food and finding the reason causing food poisoning nowadays, we have mainly
testsed the present of S. aureus, not enterotoxin which is the primary caussative agent
of Staphylococcal food poisoning. Therefore, in oder to get information of producing
enterotoxin, we carry on examining S. aureus growth and its ability to produce
enterotoxin in culture medium that contributes practically in testing food, especially in
Staphylococcal food poisoning outbreaks. S. aureus strains are cultured in TSGM and
BHI medium; then we examine their growth and test SE by ELISA method with
TECRA kit after 16, 24, 48 and 72 hours.
The results we find:
Among 36 strains surveyed, there are ten ones having ability to produce
SE (27,8%). In these ten strains, the ones from clinical samples have the highest rate
(50%).
S. aureus growth and SE amount are not correlative. SE amount
increases with time. After 16 hours culture, on TSGM, as the cell concentation reaches
7,86 log10 cfu/ml, OD ELISA value is 1,464; on BHI, as the cell concentation reaches
8,13 log10 cfu/ml OD, E
TSGM and BHI medium are the same influence for S. aureus growth
and ability to produce enterotoxin.
These S. aureus strains producce SEA, SEB, SEC. Among them, the rate
of SEA is 80%, of SEB is 10% and of SEC is 10%.









MỤC LỤC


ĐỀ MỤC TRANG

 iii
 iv
Abstract v
 vi
 ix
 x
 xi
1. MỞ ĐẦU
1.1.  1
1.2.  1
1.3.  2
2. TỔNG QUAN TÀI LIỆU
2.1. GiStaphylococcus 3
2.1.1.  3
2.1.2.  3
2.1.3.  4
2.2. Staphylococcus aureus 4
2.2.1.  4
2.2.2.  6
2.2.3.  7
2.2.4. S. aureus 8
2.3. Staphylococcus aureus 8
2.3.1. Nh 8
2.3.2. S. aureus 9
2.4. Staphylococcus aureus 11
2.4.1.  11
2.4.1.1.  11

2.4.1.2.  12
2.4.2.  13
2.5. Staphylococcus aureus 14
2.5.1.  14
2.5.2.  14
2.5.3.    16
2.6. Staphylococcus aureus 18
2.6.1.  18
2.6.2.  18
2.6.3.  19
2.6.4. 
 19
2.6.5.  20
2.6.5.1.  20
2.6.5.2.  21
2.6.6.  22
2.6.6.1.  22
2.6.6.2. - RIA 22
2.6.6.3.  23
2.6.6.4.  23
2.6.6.5. P 24
3. VẬT LIỆU VÀ PHƢƠNG PHÁP TIẾN HÀNH
3.1.   26
3.1.1.  26
3.1.2.  26
3.2.  26
3.2.1. S. aureus 26
3.2.2.  26
3.2.3.  26
3.3.  27
3.3.1.  27
3.3.2.  27

3.3.2.1. g S. aureus 27
3.3.2.2.  29
3.3.2.3. 
 30
4. KẾT QUẢ VÀ THẢO LUẬN
4.1. S. aureus 36
4.2. S. aureus 39
4.2.1.  39
4.2.1.1.  39
4.2.1.2.  40
4.2.2.  42
4.3.  44
4.3.1.  44
4.3.2.  47
4.4. SE 47
5. KẾT LUẬN VÀ ĐỀ NGHỊ
5.1.  50
5.2.  50
6. TÀI LIỆU THAM KHẢO 51
PHỤ LỤC 56













DANH SÁCH CÁC CHỮ VIẾT TẮT

BHI : Brain Heart Infusion
BP : Baird Parker
cfu : colony form unit
EIA : Enzyme Immunoassay
ELISA : Enzyme Linked Immunosorbent Assay
FAME : Fatty Acid Modifying Enzyme
KL : 
MSA : Manitol salt agar
MPN : Most Probable Number Method
MRSA : Methicilin resistant Staphylococcus aureus
PCR : Polymerase Chain Reaction
pNPP : -nitrophenyl photphatase
RIA : Radio Immunoassay
RFLP : Randomly Fragment Length Polymorphism
RLPD : Randomly Amplified Polymorphic DNA
RPLA : Reversed Passive Latex Aggulutination
S. aureus : Staphylococcus aureus
SE : Staphylococcal enterotoxin
S. epidermidis : Staphylococcus epidermidis
TSA : Tryptic soy agar
TSB : Tryptic Soy Broth
TSGM : Tecra Staphylococcal Growth Medium
TSST-1 : Toxic shock syndrom toxin
VRSA : Vancomycin Resistant Staphylococcus aureus
(+) : 
(-) : 





DANH SÁCH CÁC BẢNG

BẢNG TRANG
S. aureus, S. epidermidis Micrococci 6
S. aureus 36
S. aureus 37
S. aureus
 39
4 S. aureus
 41
5.  42
S. aureus ch 44
7. S. aureus  44
8. S. aureus  45
9S. aureus 48


















DANH SÁCH CÁC HÌNH

HÌNH TRANG
Staphylococcus aureus 4
 Staphylococcus aureus  5
3Staphylococcus aureus 14
4 S. aureus 17
nh 2.5 21
 29
2 35
3 35
S. aureus  38
2S. aureus  38
S. aureus 38
4. S. aureus  40
5. S. aureus  42
 43
7.   46
8.   46
 48
.  49

















PHẦN 1. MỞ ÐẦU

1.1. Đặt vấn đề
               Tuy

 Theo
  
s. 
Staphylococcus aureus S. aureus 

S. aureus  enterotoxin 

o
C tron
S. aureus 3-
   
 -  




S. aureus

S. aureus


 Khảo sát đậm độ và khả
năng sinh độc tố của vi khuẩn S. aureus trên môi trƣờng nuôi cấy
1.2. Mục đích
Kh s   v kh nng sinh  t c vi khu S. aureus trn
mi tr   c kho th gian kh nhau.



1.3. Nội dung nghiên cứu
o S. aureus 
o  S. aureus  
               
 Australia).
o               
S. aureus.
o 

Xem chi tiết: Khảo sát đậm độ và khả năng sinh độc tố của vi khuẩn S. aureus